Session 6A – In vitro models of toxicity testing

Session chairs

Penny Nymark, PhD, Post.Doc. at Institute of Environmental Medicine, Karolinska Instituttet, Sweden,

Martin Roursgaard, Associate Professor at Department of Public Health, University of Copenhagen, Denmark,

Session abstract

Cell-based in vitro model systems serve as animal-free alternatives for hazard evaluation of novel industrially relevant agents, such as engineered nanomaterials. In vitro models enable deep molecular and mechanistic investigation, through e.g. omics studies. In addition, in vitro models are suitable for scale-up to high-throughput screening (HTS) and high-content analysis (HCA), allowing for the screening of wide ranges of doses for several endpoints in parallel, enabling the detection of active (but non-cytotoxic) comparable doses for further study based on the most sensitive assay. Predictive toxicity modelling based on big data from omics, HTS and HCA studies provide novel ways of assessing the hazard of industrially important nanomaterials, whereas smaller scale mechanistic studies can identify hazards related to specific disease outcomes. However, to be relevant to the effects seen in vivo, in vitro models need to be validated relative the final endpoint, e.g. the adverse outcome in humans.

In this session we will hear about the current state-of-the-art of in vitro models relevant to the development of novel hazard evaluation strategies for nanosafety.

Session program

  • In vitro-based high-throughput/high content screening and omics-driven informatics support rapid and effective safety evaluation of engineered nanomaterials – Invited presentation
    Penny Nymark
  • How to make in vitro models more realistic, and compare the data to in vivo outcomes – Invited presentation
    Martin Roursgaard
  • Genotoxicity assessment of 31 nanomaterials in human bronchial epithelial cells
    Hannu Norppa
  • Assessment of adverse effects of silver nanoparticles using an air liquid interface small airway epithelial exposure model
    Chang Guo
  • Adverse effects of summer and winter ambient air in human lung cell cultures, an alternative model to assess occupational and environmental health
    Christoph Bisig
  • Dispersion and aerosolization of engineered nanoparticles in liquid suspension for in-vitro toxicity testing
    Yaobo Ding